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When one sterilizes media, how do its protein constituents remain intact? Microorganisms that were in the media are presumably destroyed by protein denaturation.
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Autoclaves are extremely effective at destroying bacteria, both pathogenic and non-pathogenic. High temperatures (usually 121 to 134 degrees Celsius) mixed with steam and pressure denature bacterial proteins, break .
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I also faced the same problem with precipitation of media after autoclave. My media consisted of calciumnitrate and potassiumdihydrogenphosphate, and they precipitated during autoclave. So .
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